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JoVE Journal Genetics
En kombinatorisk Single-cell strategi att karaktärisera molekylära och Immunophenotypic heterogenitet av mänskliga stamceller och stamceller populationer
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En kombinatorisk Single-cell strategi att karaktärisera molekylära och Immunophenotypic heterogenitet av mänskliga stamceller och stamceller populationer

Article DOI: 10.3791/57831-v • 09:34 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1
1Division of Molecular Hematology, Lund Stem Cell Center, Lund University

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Bulk gen uttryck mätningar molnet enskild cell skillnader i heterogena cellpopulationer. Här beskriver vi ett protokoll för hur encelliga gen uttryck analys och index sortering av flourescens aktiverad Cell sortering (FACS) kan kombineras för att avgränsa heterogenitet och immunophenotypically karakterisera molekylärt distinkta cellpopulationer.

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Genetik fråga 140 heterogenitet encelliga RT-qPCR FACS Index-sortering immunophenotypic karakterisering gen uttryck analys
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