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Generering av definierade genomiska modifikationer med CRISPR-CAS9 i humana pluripotenta stamceller
JoVE Journal
Genetics
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JoVE Journal Genetics
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
DOI:

09:04 min

September 25, 2019

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Chapters

  • 00:04Title
  • 01:19Test gRNA (GuidE RNA) Cutting Efficiency
  • 03:50Clone Screening
  • 04:51Transfection Setup
  • 06:07Checking for Mutations in Single Colonies
  • 07:11Results: Cutting Efficiency, and Generation and Screening of Mutations
  • 08:10Conclusion

Summary

Automatic Translation

Detta protokoll ger en metod för att underlätta generering av definierade heterozygot eller homozygot nukleotidförändringar med hjälp av CRISPR-CAS9 i humana pluripotenta stamceller.

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