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JoVE Journal Cancer Research
Live celle billeddannelse af den TGF-β/Smad3 Signaling Pathway In Vitro og In Vivo ved hjælp af en Adenovirus Reporter System
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Live celle billeddannelse af den TGF-β/Smad3 Signaling Pathway In Vitro og In Vivo ved hjælp af en Adenovirus Reporter System

Article DOI: 10.3791/57926-v • 11:06 min • July 30th, 2018
July 30th, 2018 •
*1, *1, 1, 1
1Department of Surgery (RMH), University of Melbourne
* These authors contributed equally

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Vi præsenterer her, en protokol for levende celle billeddannelse af TGF-β/Smad3 signaling aktivitet ved hjælp af en adenovirus reporter system. Dette system registrerer transcriptional aktiviteten i realtid og kan anvendes til både enkelt celler in vitro- og i levende dyrmodeller.

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Kræftforskning sag 137 TGF-β levende celle imaging adenovirus celle signalering breast cancer Smad3 transcriptional aktivering
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