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JoVE Journal Developmental Biology
Microcircuito sináptica modelado con 3D Cocultures de astrocitos y neuronas de células madre pluripotentes humanas
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Microcircuito sináptica modelado con 3D Cocultures de astrocitos y neuronas de células madre pluripotentes humanas

Article DOI: 10.3791/58034-v • 08:48 min • August 16th, 2018
August 16th, 2018 •
1, 1, 1
1Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute

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En este protocolo, nuestro objetivo es describir un método reproducible para combinar disociado humanas pluripotentes células madre derivadas neuronas y astrocitos juntos en 3D esfera cocultures, mantener estas esferas en condiciones flotantes libres y posteriormente medición de la actividad de circuito sináptico de las esferas con inmunoanálisis y matriz micropozo-microcanal grabaciones.

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Biología del desarrollo número 138 células madre neuronas astrocitos sinapsis organoide cocultivo biorreactor matriz micropozo-microcanal
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