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Journal / Immunology and Infection / Generatie van Knock-out primaire en uitgebreide…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generatie van Knock-out primaire en uitgebreide menselijke NK-cellen met behulp van Cas9 Ribonucleoproteins
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Generatie van Knock-out primaire en uitgebreide menselijke NK-cellen met behulp van Cas9 Ribonucleoproteins

Article DOI: 10.3791/58237-v • 07:26 min • June 14th, 2018
June 14th, 2018 •
1, 2, 1, 3, 1, 1, 1, 1, 1, 1, 1, 3, 1
1Center for Childhood Cancer and Blood Disease, Nationwide Children's Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, 3Center for Clinical and Translational Research, Nationwide Children's Hospital Research Institute

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Hier presenteren we een protocol om genetisch wijzigen van primaire of uitgebreide menselijke natural killer (NK) cellen met behulp van Cas9 Ribonucleoproteins (Cas9/RNPs). Met behulp van dit protocol, we genereerden menselijke NK cellen tekort voor Transformeren groeifactor-b receptor 2 (TGFBR2) en hypoxanthine phosphoribosyltransferase 1 (HPRT1).

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Immunologie en infecties probleem 136 menselijke primaire NK cellen mens uitgebreid NK-cellen CRISPR/Cas9 Cas9/RNPs gRNAs TGFBR2 TGFβ-resistente NK-cellen
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