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A Method for Labeling Vasculature in Embryonic Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Method for Labeling Vasculature in Embryonic Mice

A Method for Labeling Vasculature in Embryonic Mice

DOI:
10.3791/3267-v

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09:58 min

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October 07, 2011

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, ,
1Department of Cellular Biology,University of Georgia, 2Centre for Immunology and Infection, Department of Biology and HYMS,University of York, 3Department of Genetics,University of Georgia

Chapters

  • 00:05Title
  • 01:06Labeling Embryonic Vasculature using Fluoroscein-labeled Dextran and GSL I-isolectin B4 Facial Vein Injections and Skin Sample Preparation
  • 03:23Multi-color Labeling of Thymus Vasculature and Perivaculature Cells for Cryosections
  • 04:53Multi-color Labeling and Image Acquisition of Thymus Vasculature and Perivascular Cells for Vibratome Sections
  • 08:47Results: Vascular Labeling
  • 09:26Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a method for labeling embryonic skin and thymus blood vessels.

Tags

Labeling VasculatureEmbryonic MiceBlood Vessel NetworkOrganogenesisThymusVasculature FormationThymocyte EntryMature T-cell ExitBlood Vessel DefectsFITC-dextranGriffonia Simplicifolia Lectin IGSL 1 – Isolectin B4CD31 Antibody StainingThymic Perivascular MesenchymeCryosectionsVibratome SectionsThymus Vascular DefectsTEC-derived MoleculesVascular NetworksMultiple Organs

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