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Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
JoVE Journal
Medicine
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JoVE Journal Medicine
Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
DOI:

09:54 min

November 04, 2018

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Chapters

  • 00:04Title
  • 01:22Laparoscopic Partial Hepatectomy
  • 03:28Hepatocyte Isolation and Transplantation
  • 07:24Results: Representative Lentiviral Vector-mediated Hepatocyte Liver Function Rescue
  • 09:09Conclusion

Summary

Automatic Translation

This protocol is intended to describe porcine hepatocyte isolation and ex vivo gene delivery to cure models of metabolic diseases via autologous cell transplantation. Although this particular model enjoys unique advantages that favor successful therapy, the application is a relevant foundation to address additional diseases and indications.

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