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Journal / Biochemistry / Et Fluorogenic peptid kavalergang Assay til at…
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Biochemistry

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JoVE Journal Biochemistry
Et Fluorogenic peptid kavalergang Assay til at screene for proteolytiske aktivitet
Measuring Protein Binding to F-actin by Co-sedimentation
Journal (Biochemistry)
Measuring Protein Binding to F-actin by Co-sedimentation
High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography (Video) | JoVE
Journal (Biochemistry)
High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography (Video) | JoVE
 
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Et Fluorogenic peptid kavalergang Assay til at screene for proteolytiske aktivitet: ansøgninger om coronavirus spike protein aktivering

Article DOI: 10.3791/58892-v • 07:53 min • January 9th, 2019
January 9th, 2019 •
*1,2, *1,3, 1,4, 1, 1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 3Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, 4Department of Cell Biology and Molecular Genetics, University of Maryland
* These authors contributed equally

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Vi præsenterer en fluorogenic peptid kavalergang assay, der giver mulighed for en hurtig screening af proteaser proteolytiske aktivitet på peptider der repræsenterer webstedet spaltning af viral fusion peptider. Denne metode kan også bruges på andre aminosyre motiv i en protein sekvens til at teste for protease aktivitet.

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Biokemi spørgsmålet 143 peptid assay Coronavirus Feline coronavirus MERS-CoV Fusion peptid Protease kavalergang Furin
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