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Journal / Genetics / Genomet redigering i däggdjur cellinjer med…
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Genetics

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JoVE Journal Genetics
Genomet redigering i däggdjur cellinjer med CRISPR-Cas
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
Journal (Genetics)
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Journal (Genetics)
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
 
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Genomet redigering i däggdjur cellinjer med CRISPR-Cas

Article DOI: 10.3791/59086-v • 07:56 min • April 11th, 2019
April 11th, 2019 •
1, 1, 1,2, 1,2
1Genome Institute of Singapore, Agency for Science Technology and Research, 2School of Chemical and Biomedical Engineering, Nanyang Technological University

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CRISPR-Cas är en kraftfull teknik för att konstruera komplexa genomen hos växter och djur. Här, detalj vi ett protokoll för att effektivt redigera det mänskliga genomet som använder olika Cas endonucleases. Vi belysa viktiga överväganden och konstruktionsparametrar att optimera redigering effektivitet.

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Genetik fråga 146 CRISPR Cas9 Cas12a editering icke-homolog slutet att gå homologi-regisserad reparation
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