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Fluorescerende visualisering af mango-Tagged RNA i polyacrylamid gels via en Poststaining metode
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Fluorescerende visualisering af mango-Tagged RNA i polyacrylamid gels via en Poststaining metode

Article DOI: 10.3791/59112-v • 06:06 min • June 21st, 2019
June 21st, 2019 •
*1, *1, 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University
* These authors contributed equally

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Her præsenterer vi en følsom, hurtig og diskriminerende post-gel farvning metode til billede RNAs mærket med RNA mango aptamers I, II, III, eller IV, ved hjælp af enten indfødte eller denaturerende polyacrylamid gel elektroforese (PAGE) gels. Efter at have kørt standard side gels, mango-Tagged RNA kan let farves med TIL1-biotin og derefter analyseres ved hjælp af almindeligt tilgængelige fluorescens læsere.

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Biokemi RNA mango fluorescens Denaturerings gels indfødte gels gel farvning detekterings metode PAGE
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