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Forbedret gær One-hybrid skærme til at identificere transskription faktor Binding til menneskelige DNA-sekvenser
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Forbedret gær One-hybrid skærme til at identificere transskription faktor Binding til menneskelige DNA-sekvenser

Article DOI: 10.3791/59192-v • 11:25 min • February 11th, 2019
February 11th, 2019 •
*1, *1, *1, 1,2
1Department of Biology, Boston University, 2Bioinformatics Program, Boston University
* These authors contributed equally

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Vi præsenterer her, en forbedret gær one-hybrid screening protokol for at identificere de transkriptionsfaktorer (TFs), der kan bindes til en menneskelig DNA region af interesse. Denne metode bruger en høj overførselshastighed screening rørledning, der kan forhøre bindingen af > 1.000 TFs i et enkelt eksperiment.

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Genetik spørgsmålet 144 gær one-hybrid Y1H transskription faktor menneskelige gen forordning DNA eY1H
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