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Journal / Genetics / 使用基因激活 crispr 过度表达长非编码…
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Genetics

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JoVE Journal Genetics
使用基因激活 crispr 过度表达长非编码 rna
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使用基因激活 crispr 过度表达长非编码 rna

Article DOI: 10.3791/59233-v • 13:04 min • March 1st, 2019
March 1st, 2019 •
1, 1, 2, 1, 3, 3, 1, 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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传统的基于 cdna 的过度表达技术由于具有潜在功能的多个拼接形式, 对长非编码 rna 的过度表达的适用性有限。本综述报告了一个协议使用 crispr 技术超细的多个拼接变体的长非编码 rna。

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遗传学 第145期 而英卡 crispr ifng-as1 过度表达 慢病毒 实时 lncRNA
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