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使用基因组-规模CRISPR/Cas9基因屏幕进行细胞表面受体识别

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Please note that all translations are automatically generated. Click here for the English version.

使用基因组-规模CRISPR/Cas9基因屏幕进行细胞表面受体识别

DOI:

10.3791/60803-v

•

08:49 min

•

June 06, 2020

•

Sumana Sharma², Gavin J. Wright

¹Cell Surface Signalling Laboratory,Wellcome Trust Sanger Institute, ²EMBL-EBI,Wellcome Genome Campus

Chapters

00:04Introduction
01:07Quantification and Oligomerization of Monomeric Biotinylated Protein
03:26Genetic Screening for Cell Surface Binding
05:13Bioinformatics Analysis to Identify the Receptor and Related Pathways
06:05Results: Genetic Screens for the Identification of Cell Surface Binding Partners
07:57Conclusion

Summary

Automatic Translation

本手稿描述了一种基于基因组规模的细胞筛选方法，用于识别细胞外受体-配体相互作用。

Tags

Keywords: Cell Surface Receptor CRISPR/Cas9 Genetic Screens Cell Signaling Cell Recognition Receptor-ligand Interactions Cell Surface Molecules Therapeutic Implications High-throughput Cell Sorting Next-generation Sequencing Biotinylated Proteins Streptavidin-PE Dilution Buffer Wash Buffer Alkaline Phosphatase Conjugate

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