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Genetics
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Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira
 

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

Article DOI: 10.3791/62631-v
August 14th, 2021

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Summary August 14th, 2021

Here, the application of CRISPR interference (CRISPRi) for specific gene silencing in Leptospira species is described. Leptospira cells are transformed by conjugation with plasmids expressing dCas9 (catalytically "dead" Cas9) and a single-guide RNA (sgRNA), responsible for base pairing to the desired genomic target. Methods to validate gene silencing are presented.

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