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Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis
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Chapters
- 00:04Title
- 00:325-Fluorouracil (5-FU) Mucositis Induction
- 01:095-Bromo-2 -Deoxyuridine (BrdU) Quantification and Tissue Collectio
- 02:42BrdU Immunoreactive Cell Area Measurement
- 04:23Crypt Depth Measurement
- 04:57Results: Representative Small Intestine Injury Recovery Assessment
- 06:17Conclusion
Here, we present a protocol to establish important endpoints and proliferative markers of small intestinal injury and compensatory hyperproliferation using a model of chemotherapy-induced mucositis. We demonstrate the detection of proliferating cells using a cell cycle specific marker and using small intestinal weight, crypt depth, and villus height as endpoints.
