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JoVE Journal Biochemistry
In vitro-allestedsnærværende og Deubiquitinationassays af Nukleosomale histoner
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In vitro-allestedsnærværende og Deubiquitinationassays af Nukleosomale histoner

Article DOI: 10.3791/59385-v • 11:36 min • July 25th, 2019
July 25th, 2019 •
*1, *1, 1, 1, 1, 1, 1, 1,2, 1,2
1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System
* These authors contributed equally

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Allestedsnærværende er en post-translationel modifikation, der spiller en vigtig rolle i cellulære processer og er tæt koordineret af deubiquitination. Defekter i begge reaktioner ligger til grund for humane patologier. Vi leverer protokoller til gennemførelse af allestedsnærværende og deubiquitination reaktion in vitro ved hjælp af rensede komponenter.

Tags

Biokemi allestedsnærværende deubiquitination E3 ubiquitin Ligase PRC1 deubiquitinase PR-DUB Chromatin BAP1 ASXL BMI1 RING1B H2A
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