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Journal / Biochemistry / Funktionel karakterisering af RING type E3…
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Biochemistry

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JoVE Journal Biochemistry
Funktionel karakterisering af RING type E3 Ubiquitin-Ligaser in vitro og i planta
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Funktionel karakterisering af RING type E3 Ubiquitin-Ligaser in vitro og i planta

Article DOI: 10.3791/60533-v • 10:27 min • December 5th, 2019
December 5th, 2019 •
1, 2, 2, 2, 2, 1, 2
1Department of Entomology, Plant Pathology and Nematology, University of Idaho, 2Department of Plant Sciences, University of Idaho

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Formålet med dette manuskript er at fremlægge en skitse til de omfattende biokemiske og funktionelle undersøgelser af RING typen E3 ubiquitin-ligaser. Denne flertrinsrørledning, med detaljerede protokoller, validerer en enzymatisk aktivitet af det testede protein og demonstrerer, hvordan aktiviteten knyttes til funktion.

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Biokemi allestedsnærværende RING-type E3 ubiquitin Ligase RHA1B Effector Globodera pallida Nicotiana benthamiana Agrobacterium-medieret protein forbigående udtryk site-instrueret mutagenese
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