Journal
/
/
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
DOI:

11:24 min

May 14, 2019

, , , , ,

Chapters

  • 00:04Title
  • 00:42Overnight Culture of Human Islets for Recovery After Shipment
  • 01:32Preparation of Single Cell Suspension from Human Islets
  • 04:03Pseudoislet Formation and Transduction by Lentivirus
  • 08:44Results: Morphological and Functional Studies Using Gene Modified Human Pseudoislets
  • 09:53Conclusion

Summary

Automatic Translation

A protocol to create gene modified human pseudoislets from dispersed human islet cells that are transduced by lentivirus carrying short hairpin RNA (shRNA) is presented. This protocol utilizes readily available enzyme and culture vessels, can be performed easily, and produces genetically modified human pseudoislets suitable for functional and morphological studies.

Related Videos

Read Article