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In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
DOI:

09:04 min

May 11, 2020

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Chapters

  • 00:04Introduction
  • 01:08Microplate Sensitization
  • 02:07Microplate Passivation
  • 02:33ELISA Assay
  • 06:44Results: Analysis of the ELISA Test
  • 08:00Conclusion

Summary

Automatic Translation

Here we describe an indirect ELISA sandwich immunocapture to determine the immunogenic glycoprotein contents in rabies vaccines. This test uses a neutralizing Monoclonal Antibody (mAb-D1) recognizing glycoprotein trimers. It is an alternative to the in vivo NIH test to follow the consistency of vaccine potency during production.

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