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Developmental Biology

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JoVE Journal Developmental Biology
Hurtig og effektiv ekspression af flere proteiner i aviær embryoner ved hjælp af mRNA-elektroporation
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Hurtig og effektiv ekspression af flere proteiner i aviær embryoner ved hjælp af mRNA-elektroporation

Article DOI: 10.3791/59664-v • 09:40 min • June 7th, 2019
June 7th, 2019 •
1, 2,3, 1,2,3
1Department of Biological Sciences, University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles

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Vi rapporterer Messenger RNA (mRNA) elektroporation som en metode, der tillader hurtig og effektiv ekspression af flere proteiner i vagtler embryo modelsystemet. Denne metode kan anvendes til fluorescently label celler og registrere deres in vivo bevægelser ved time-lapse mikroskopi kort efter elektroporation.

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Udviklingsmæssige biologi mRNA elektroporation time-lapse mikroskopi vagtler aviær embryo FRAP
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