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Inducing Complete Polyp Regeneration from the Aboral Physa of the Starlet Sea Anemone Nematostella vectensis
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Chapters
- 00:05Title
- 01:05Selection and Relaxation of Nutritionally Conditioned Animals
- 02:12Amputation
- 03:44Assessing Regeneration with the Nematostella Regeneration Staging System (NRSS)
- 05:47Results: Effects of Chloroquine, and Inhibitor of Autophagy
- 06:35Conclusion
Here we demonstrate how to induce and monitor regeneration in the Starlet Sea Anemone Nematostella vectensis, a model cnidarian anthozoan. We demonstrate how to amputate and categorize regeneration using a morphological staging system, and we use this system to reveal a requirement for autophagy in regenerating polyp structures.
