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Journal / Genetics / Gene direto knock-out de células-tronco neurais…
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JoVE Journal Genetics
Gene direto knock-out de células-tronco neurais da medula espinhal Axolotl via eletroporação de complexos de proteína-gRNA CAS9
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Gene direto knock-out de células-tronco neurais da medula espinhal Axolotl via eletroporação de complexos de proteína-gRNA CAS9

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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É apresentado aqui um protocolo para executar a batida-para fora do gene tempo-e espaço-restrito em cordas espinais do Axolotl injetando o complexo de CAS9-gRNA no canal central da medula espinal seguido pelo Electroporation.

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Genética edição 149 Axolotl medula espinhal regeneração CRISPR-Cas9 células-tronco neurais nocaute genético
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