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Desenvolvimento de linhas de células musculares knock-out usando edição de genes CRISPR/Cas9 mediados por lentivírus
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Desenvolvimento de linhas de células musculares knock-out usando edição de genes CRISPR/Cas9 mediados por lentivírus

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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O protocolo descreve como gerar myoblasts knock-out usando CRISPR/Cas9, desde o design de guide-RNAs até a clonagem celular e caracterização dos clones knock-out.

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Genética Edição 184
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