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Journal / Chemistry / 基于NMR的活性测定,用于测定核苷裂解酶的化合物抑制、IC50值、伪影活性和全细胞活性…
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JoVE Journal Chemistry
基于NMR的活性测定,用于测定核苷裂解酶的化合物抑制、IC50值、伪影活性和全细胞活性
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Journal (Chemistry)
Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
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基于NMR的活性测定,用于测定核苷裂解酶的化合物抑制、IC50值、伪影活性和全细胞活性

Article DOI: 10.3791/59928-v • 10:24 min • June 30th, 2019
June 30th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,2, 1,3
1Department of Chemistry, Adelphi University, 2Department of Chemistry, Washington University in St. Louis, 3Department of Chemistry, Boston University

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基于NMR的活性测定已经开发出来,以识别和表征两种核苷糖酶的抑制剂。为500μM和250μM的初始化合物测定、确定IC50值的剂量反应测定、洗涤剂计数器屏幕测定、跳跃稀释计数器筛查测定以及大肠杆菌全细胞测定提供了协议。

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化学 问题 148 洗涤剂计数器屏幕 酶活性测定 IC50值 跳跃稀释 1H NMR 19F NMR 全细胞 NMR 核苷核糖核酸酶
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