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Neuroscience

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JoVE Journal Neuroscience
Analyse mitokondriel transport og morfologi i Human Induceret pluripotente stamceller-afledte neuroner i arvelige spastiske paraplegi
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Analyse mitokondriel transport og morfologi i Human Induceret pluripotente stamceller-afledte neuroner i arvelige spastiske paraplegi

Article DOI: 10.3791/60548-v • 07:32 min • February 9th, 2020
February 9th, 2020 •
1,2, 1, 1, 3, 1,2
1Department of Biomedical Sciences, University of Illinois College of Medicine Rockford, 2Department of Bioengineering, University of Illinois at Chicago, 3MD Program, University of Illinois College of Medicine Rockford

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Nedsat mitokondrielle transport og morfologi er involveret i forskellige neurodegenerative sygdomme. Den præsenterede protokol bruger inducerede pluripotente stamceller-afledte forhjernen neuroner til at vurdere mitokondrielle transport og morfologi i arvelig spastisk paraplegi. Denne protokol giver mulighed for karakterisering af mitokondriel handel langs axoner og analyse af deres morfologi, som vil lette studiet af neurodegenerative sygdomme.

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Neurovidenskab mitokondriel transport mitokondriel morfologi forhjernen neuroner inducerede pluripotente stamceller axonal degeneration arvelig spastisk paraplegi
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