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Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
JoVE Journal
Biology
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JoVE Journal Biology
Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi

DOI:
10.3791/60613-v

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07:44 min

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November 28, 2019

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,
1Department of Microbiology,University of Innsbruck

Chapters

  • 00:04Title
  • 01:06Preparation of Fungal Samples
  • 01:40Sample Preparation for Microscopy
  • 02:57Live-cell Microscopy
  • 04:16Results: Live-Cell Imaging of Filamentous Fungi
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

Vital fluorescent dyes are essential tools for live-cell imaging analyses in modern fungal cell biology. This paper details the application of established and lesser-known fluorescent dyes for tracking plasma membrane dynamics, endo-/exocytosis and cell wall morphogenesis in filamentous fungi.

Tags

Membrane DyesCell Wall DyesLive-cell ImagingFilamentous FungiFungal CellsOrganelle DynamicsVital StainingImage-guided DiagnosisAspergillosisDrug Uptake DynamicsSample PreparationMicroscopyImage Acquisition Settings

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