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Journal / Developmental Biology / Periaortik Yağ Dokusundan Nöral Adipoz-Türetilmiş …
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Developmental Biology

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JoVE Journal Developmental Biology
Periaortik Yağ Dokusundan Nöral Adipoz-Türetilmiş Kök Hücrelerin İzolasyon, Kültür ve Adipojenik İndüksiyonu
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
Journal (Developmental Biology)
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Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
Journal (Developmental Biology)
Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
 
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Periaortik Yağ Dokusundan Nöral Adipoz-Türetilmiş Kök Hücrelerin İzolasyon, Kültür ve Adipojenik İndüksiyonu

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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Wnt-1 Cre+/-periaortik yağ dokusundan elde edilen adipoz türetilmiş kök hücrelerin (NCADSCs) izolasyonu, kültürü ve adipojenik indüksiyonu için bir protokol salıyoruz. Rosa26RFP/+ fareler. NCADSCs in vitro adipogenez veya lipogenez modelleme için ADSCs kolay erişilebilir bir kaynak olabilir.

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Gelişimbiyolojisi Sayı 157 nöral krest hücresi Wnt-1 fare periaortik yağ dokusu yağ türevi stromal hücreler stromal vasküler fraksiyon hücre kültürü adipojenik indüksiyon
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