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Journal / Bioengineering / Crescimento de Camadas de Células Endoteliais De…
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JoVE Journal Bioengineering
Crescimento de Camadas de Células Endoteliais De Córnea Humana e Ovelha em Membranas Biomateriais
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Crescimento de Camadas de Células Endoteliais De Córnea Humana e Ovelha em Membranas Biomateriais

Article DOI: 10.3791/60762-v • 05:20 min • February 6th, 2020
February 6th, 2020 •
1, 2, 1, 1,3,4,5,6,7,8, 1,3,4
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Science and Engineering Faculty, Queensland University of Technology, 6Faculty of Medicine, University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 8Faculty of Science, University of Western Australia

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Este protocolo descreve os passos críticos necessários para estabelecer e cultivar culturas de células endoteliais da córnea a partir de explantas de tecido humano ou ovino. Também é apresentado um método para subculindo células endoteliais córneas em biomateriais membranrosas.

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Bioengenharia Edição 156 endotelio córnea córnea membrana do Descemet explanta membrana biomaterial câmara de cultura
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