/
/
Crescimento de Camadas de Células Endoteliais De Córnea Humana e Ovelha em Membranas Biomateriais
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health,Queensland University of Technology, 4Institute of Health and Biomedical Innovation,Queensland University of Technology, 5Science and Engineering Faculty,Queensland University of Technology, 6Faculty of Medicine,University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 8Faculty of Science,University of Western Australia
Chapters
- 00:05Introduction
- 00:35Explant Dissection and Cell Culture Procedure
- 01:52Growth of Corneal Endothelial Cell Layers on Biomaterial Membranes
- 03:28Results: Corneal Endothelial Cell Cultures Derived from Sheep and Human Explants
- 04:47Conclusion
Este protocolo descreve os passos críticos necessários para estabelecer e cultivar culturas de células endoteliais da córnea a partir de explantas de tecido humano ou ovino. Também é apresentado um método para subculindo células endoteliais córneas em biomateriais membranrosas.
