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Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
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Bioengineering
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JoVE Journal Bioengineering
Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

DOI:
10.3791/60762-v

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05:20 min

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February 06, 2020

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, , , ,
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health,Queensland University of Technology, 4Institute of Health and Biomedical Innovation,Queensland University of Technology, 5Science and Engineering Faculty,Queensland University of Technology, 6Faculty of Medicine,University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 8Faculty of Science,University of Western Australia

Chapters

  • 00:05Introduction
  • 00:35Explant Dissection and Cell Culture Procedure
  • 01:52Growth of Corneal Endothelial Cell Layers on Biomaterial Membranes
  • 03:28Results: Corneal Endothelial Cell Cultures Derived from Sheep and Human Explants
  • 04:47Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the critical steps required to establish and grow corneal endothelial cell cultures from explants of human or sheep tissue. A method for subculturing corneal endothelial cells on membranous biomaterials is also presented.

Tags

Keywords: Corneal Endothelial CellsCell CultureBiomaterial MembranesDescemet’s MembraneMicro Boyden ChamberEpithelial To Mesenchymal TransitionCell IsolationCell ExpansionCell SubculturePrimary CellsAttachment FactorCulture MediumCell Suspension

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