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JoVE Journal
Bioengineering

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Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
 

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Article DOI: 10.3791/60762-v
February 6th, 2020

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Summary February 6th, 2020

This protocol describes the critical steps required to establish and grow corneal endothelial cell cultures from explants of human or sheep tissue. A method for subculturing corneal endothelial cells on membranous biomaterials is also presented.

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