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Chapters
- 00:04Introduction
- 00:43System Setup
- 01:05Fly Preparation
- 01:47Video Generation for Feature Learning Leg Segmentation and Tracking (FLLIT) Analysis
- 02:52FLLIT Installation
- 03:50Automated Leg Tracking
- 05:13Data Processing and Video Generation
- 05:54Representative Results: Automated Leg Movement Tracking in Freely-Moving Insects
- 07:31Conclusion
We describe detailed protocols for using FLLIT, a fully automated machine learning method for leg claw movement tracking in freely moving Drosophila melanogaster and other insects. These protocols can be used to quantitatively measure subtle walking gait movements in wild type flies, mutant flies and fly models of neurodegeneration.
