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A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
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Bioengineering
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JoVE Journal Bioengineering
A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

DOI:
10.3791/61076-v

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08:28 min

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May 21, 2020

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, , ,
1Department of Biomedical Engineering,The University of Akron

Chapters

  • 00:04Introduction
  • 00:45PDMS Fabrication
  • 02:15Chip Assembly and Sterilization
  • 03:47Mechanical Loading Device Assembly
  • 04:33Loading Experiment
  • 06:23Results: Functional Activity and Effects of Mechanically Induced Strain
  • 07:48Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present protocols for analyzing bone remodeling within a lab-on-a-chip platform. A 3D printed mechanical loading device can be paired with the platform to induce osteocyte mechanostransduction by deforming the cellular matrix. The platform can also be used to quantify bone remodeling functional outcomes from osteoclasts and osteoblasts (resorption/formation).

Tags

Lab-on-a-chipOsteocyteMechanotransductionBone RemodelingPDMSMicrofluidicsBone Cell CultureMechanical LoadingOsteoporosisFracture HealingPeriprosthetic Osteolysis

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