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Biology

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Neutrophil Isolation Protocol
 
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Neutrophil Isolation Protocol

Article DOI: 10.3791/745-v 07:13 min July 23rd, 2008
July 23rd, 2008

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Summary

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Click here for the English version.

Neutrophils are among the first cells to arrive on the site of inflammatory immune response, and their functions and mechanisms have been studied extensively in vitro. We demonstrate a standard density gradient separation method to isolate human neutrophils from whole blood using commercially available separation media.

Tags

Neutrophil Isolation PMN Isolation Leukocytes Inflammatory Immune Response Neutrophil Functions Clean Method Fast Method Reliable Method Density Gradient Separation Sodium Metrizoate Dextran 500 Layering Whole Blood Centrifugation Lysis Of Erythrocytes Cell Counting Viability Assessment
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