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An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation
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An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation

An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation

DOI:
10.3791/61812-v

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05:42 min

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October 25, 2021

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, ,
1Department of Pediatrics,Johns Hopkins University School of Medicine, 2Department of Physiology,Temple University School of Medicine, 3Department of Pediatrics,Seattle Children’s Hospital

Chapters

  • 00:04Introduction
  • 00:45Procedure
  • 03:08Results: Purity and Activity Levels of the Isolated Primary Hepatocytes
  • 05:08Conclusion

Summary

Automatic Translation

Automatic Translation

Primary hepatocytes are a valuable tool to study liver response and metabolism in vitro. Utilizing commercially available reagents, an improved time- and labor-efficient protocol for mouse primary hepatocyte isolation was developed.

Tags

Keywords: Mouse Primary HepatocytesLiver StudiesGlucose MetabolismHepatic Drug TestingTime-efficientLabor-efficientProtocolPeristaltic PumpPerfusion MediumCollagenase Dispase MediumWash MediumCell StrainerHBSSCentrifugationCell Culture

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