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Titration of Human Coronaviruses Using an Immunoperoxidase Assay
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Titration of Human Coronaviruses Using an Immunoperoxidase Assay

Article DOI: 10.3791/751-v • 12:00 min • April 28th, 2008
April 28th, 2008 •
1, 1, 1, 1
1Laboratory of Neuroimmunovirology, INRS-Institut Armand-Frappier

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In this video, we demonstrate an alternative method for detection and titering of viruses using an enzymatic antigen detection technique known as an immunoperoxidase assay. Here, we will show you how to collect your viral samples, prepare the cells for testing, and finally the immunoperoxidase assay using serial dilutions to determine the viral titer.

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Titration Human Coronaviruses Immunoperoxidase Assay Viral Titers Virologists Plaque Assays Cytopathic Effects Prototype Strains 229E And OC43 Alternative Assay Indirect Immunoperoxidase Assay (IPA) Virus Detection 96-well Plate Format TCID50 Tissue Culture Infectious Dose 50 Percent Reliable Method HCoV-229E HCoV-OC43 Biological Samples
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