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Chapters
- 00:05Title
- 01:41Transcriptional Silencing of ASM
- 04:18Preparation of Reagents for Live Microscopy
- 05:29Live Cell Imaging of FM1-43 Influx into SLO Treated and Untreated Cells
- 10:21Quantification of FM1-43 Influx into Cells
- 14:03Results: Ca2+ and Sphingomyelinase are Involved in Plasma Membrane Repair
- 17:34Conclusion
Live imaging of cells exposed to the lipophilic dye FM1-43 allows precise determination of the kinetics by which pore-forming toxins are removed from the plasma membrane. This is a sensitive assay that can be used to assess requirements for Ca2+, sphingomyelinase and other factors on plasma membrane repair.
