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Culturing Microglia from the Neonatal and Adult Central Nervous System
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Culturing Microglia from the Neonatal and Adult Central Nervous System

Culturing Microglia from the Neonatal and Adult Central Nervous System

27,251 Views

11:28 min

August 09, 2013

DOI:

11:28 min
August 09, 2013

28 Views
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Summary

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We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.

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