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钙火花的评估在正常肌肉纤维

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

JoVE 杂志
生物学

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JoVE 杂志生物学

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

钙火花的评估在正常肌肉纤维

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15,070 Views

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11:22 min

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February 24, 2014

DOI:

10.3791/50898-v

DOI:

10.3791/50898-v

•

11:22 min

February 24, 2014

•

2 Views

Ki Ho Park¹, Noah Weisleder², Jingsong Zhou³, Kristyn Gumpper¹, Xinyu Zhou¹, Pu Duann⁴, Jianjie Ma¹, Pei-Hui Lin¹

¹Department of Surgery, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center, ²Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center, ³Department of Molecular Biophysics and Physiology,Rush University Medical Center, ⁴Department of Internal Medicine, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center

Summary

这里描述的是一种方法来直接测量钙火花的Ca ^{2 +}的基本单元从正常肌肉纤维肌质网释放。该方法利用渗透压应激介导的触发的Ca ^{2 +}释放在孤立的肌纤维兰尼碱受体。动力学和细胞内Ca ^{2 +}信号的稳态容量可以被用来评估在健康和疾病中的肌肉功能。

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