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Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Article DOI: 10.3791/64665-v • 05:49 min • December 2nd, 2022
December 2nd, 2022 •
*1, *2, 1, 3, 1,3, 1,2
1Graduate Program in Bioengineering, University of California, Berkeley and University of California, San Francisco, 2Department of Mechanical Engineering, University of California, Berkeley, 3Department of Electrical Engineering and Computer Sciences, University of California, Berkeley
* These authors contributed equally

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Presented here is a method to mechanically phenotype single cells using an electronics-based microfluidic platform called mechano-node-pore sensing (mechano-NPS). This platform maintains moderate throughput of 1-10 cells/s while measuring both the elastic and viscous biophysical properties of cells.

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Mechano-node-pore Sensing Label-free Platform Multi-parameter Single-cell Viscoelastic Measurements Electronics-based Microfluidic Platform Single Cell Elastic Properties Single Cell Viscous Properties Minimal Sample Preparation Electronic Measurement Non-destructive Approach Downstream Analyses Cell Behavior Disease Progression Drug Response Plasma Treated Components Methanol And Deionized Water Solution Glass Substrate PDMS Mold Device Fabrication Pressure Source PCB Benchtop Hardware Data Acquisition Software Clamps Header Pins
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