Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Sylvia T. Cheung; Soroush Shakibakho; Eva Y. So; Alice L-F Mui

doi:10.3791/52807

Journal

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Biologie

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Transfizieren RAW264.7-Zellen mit einem Luciferase-Reportergen

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

JoVE Journal
Biologie

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JoVE Journal Biologie

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Transfizieren RAW264.7-Zellen mit einem Luciferase-Reportergen

Please note that all translations are automatically generated. Click here for the English version.

20,529 Views

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12:58 min

•

June 18, 2015

DOI:

10.3791/52807-v

DOI:

10.3791/52807-v

•

12:58 min

June 18, 2015

•

18 Views

Sylvia T. Cheung^1,2,3, Soroush Shakibakho^1,2, Eva Y. So^1,2, Alice L-F Mui^1,2,3

¹Immunity and Infection Research Centre,Vancouver Costal Health Research Institute, ²Department of Surgery,University of British Columbia, ³Department of Biochemistry and Molecular Biology,University of British Columbia

Summary

Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.

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Transfizieren RAW264.7-Zellen mit einem Luciferase-Reportergen

Summary

Automatically generated

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