JoVE Journal
Biochemistry
Biochemistry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
DNA methyltransferases are potential cancer drug targets. Here, a protocol is presented to assess small molecules for DNA methyltransferase inhibition. This assay utilizes an endonuclease to couple DNA methylation to fluorescence generation and allows for enzyme activity to be monitored in real time.