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DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
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Biochemistry
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JoVE Journal Biochemistry
DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

DOI:
10.3791/56528-v

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08:11 min

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January 28, 2018

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, ,
1Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas,Universidad de Chile

Chapters

  • 00:00Title
  • 00:31Staining
  • 02:56DNA Recovery and Integration Comparison
  • 05:49Results
  • 06:53Discussion

Summary

Automatic Translation

Automatic Translation

This method allows selective staining and quantification of DNA in gels by soaking the gel in a SYBR Green I/Nitro Blue Tetrazolium solution and then exposing it to sunlight or a blue light source. This produces a visible precipitate and requires almost no equipment, making it ideal for field use.

Tags

Keywords: DNA StainingFormazan PrecipitationBlue Light ExposureSYBR Green INitro Blue Tetrazolium (NBT)Polyacrylamide GelAgarose GelElectrophoresisBand QuantificationCalibration CurvePlasmidChemical TransformationCompetent CellsAmpicillin

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