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Tovejs Retroviral Integration Site PCR Metode og Quantitative Data Analysis Workflow

Article DOI: 10.3791/55812-v • 12:53 min • June 14th, 2017
June 14th, 2017 •
*1,2, *3, 1, 3, 4, 1,5, 6
1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)
* These authors contributed equally

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Dette manuskript beskriver den eksperimentelle procedure og softwareanalyse for et tovejs integrationssite-assay, der samtidigt kan analysere opstrøms og nedstrøms vektor-værtsforbindelses-DNA. Tovejsede PCR-produkter kan anvendes til enhver downstream-sekventeringsplatform. De resulterende data er nyttige til en høj-gennemløbs kvantitativ sammenligning af integrerede DNA-mål.

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Genetik Udgave 124 Retrovirus Integrationssteder Næste generations sekventering Retroviral genterapi Stamceller Cancers Cellular barcoding Bioinformatics
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