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Directe herprogrammering van menselijke fibroblasten in myoblasten om therapieën voor neuromusculaire aandoeningen te onderzoeken

Article DOI: 10.3791/61991-v • 10:28 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1, 2, 2,3,4,6, 5, 1,6, 1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University

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Dit protocol beschrijft de omzetting van huidfibbroblasten in myoblasten en hun differentiatie in myotubes. De cellijnen zijn afgeleid van patiënten met neuromusculaire aandoeningen en kunnen worden gebruikt om pathologische mechanismen te onderzoeken en therapeutische strategieën te testen.

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Genetica Herprogrammering hTERT MyoD fibroblast myoblast neuromusculaire stoornis therapie testen
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