Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Huiqin Tang; Hui Xie; Zhun Wang; Shuanghe Peng; Wenli Ni; Linpei Guo

doi:10.3791/63125

Protocolo económico y eficiente para aislar y cultivar células dendríticas derivadas de la médula...

JoVE Journal
Immunology and Infection
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Protocolo económico y eficiente para aislar y cultivar células dendríticas derivadas de la médula ósea de ratones

Full Text

11,367 Views

04:29 min

July 1, 2022

DOI: 10.3791/63125-v

Huiqin Tang*¹, Hui Xie*¹, Zhun Wang¹, Shuanghe Peng², Wenli Ni¹, Linpei Guo³

¹Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, ²Department of Pathology,The Second Hospital of Tianjin Medical University, ³Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This article presents an economical and efficient method for isolating and generating high-purity bone marrow-derived dendritic cells from mice. The protocol involves a 7-day culture with GM-CSF/IL-4.

Key Study Components

Area of Science

Neuroscience
Immunology
Cell Biology

Background

Dendritic cells (DCs) are crucial for tumor immunity research.
DCs are rare in tissues, making their isolation challenging.
Traditional methods for DC differentiation are complex and costly.
This study aims to simplify the process of generating DCs from bone marrow.

Purpose of Study

To develop a cost-effective method for isolating DCs.
To improve the efficiency of DC generation from mouse bone marrow.
To support research in tumor immunity by providing high-purity DCs.

Methods Used

Isolation of bone marrow from mice.
Culture of bone marrow cells with GM-CSF/IL-4.
Assessment of cell purity and viability.
Approval from the Institutional Animal Ethics Committee.

Main Results

Successful isolation of high-purity bone marrow-derived DCs.
Demonstrated efficiency of the new protocol.
Potential for broader application in tumor immunity research.
Reduction in time and cost compared to traditional methods.

Conclusions

The developed method is a viable alternative for DC generation.
This approach can facilitate further research in immunology.
Future studies may explore applications in various therapeutic contexts.

Frequently Asked Questions

What are dendritic cells?

Dendritic cells are immune cells that play a key role in presenting antigens and activating T-cells.

Why is it important to isolate dendritic cells?

Isolating dendritic cells is crucial for studying their role in immune responses and developing immunotherapies.

What is GM-CSF/IL-4?

GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) and IL-4 (Interleukin-4) are cytokines used to promote the differentiation of bone marrow cells into dendritic cells.

How does this method compare to traditional methods?

This method is more economical and efficient, reducing both time and costs associated with traditional DC isolation techniques.

What ethical considerations are involved in this study?

The study was approved by the Institutional Animal Ethics Committee, ensuring humane treatment of animal subjects.

Aquí, presentamos un método económico y eficiente para aislar y generar células dendríticas derivadas de la médula ósea de alta pureza a partir de ratones después de 7 días de cultivo con 10 ng / ml GM-CSF / IL-4.

Protocolo económico y eficiente de aislamiento y generación de células dendríticas derivadas de la médula ósea a partir de ratón. Los procedimientos que involucran sujetos animales han sido aprobados por el Comité de Ética Animal de la Institución de la Universidad Médica de Nanjing.One.Introduction. Con el aumento en la investigación de la inmunidad tumoral, la demanda de células DC está aumentando gradualmente.

Sin embargo, los DC son raros en todos los tejidos. El método tradicional de inducir principalmente la diferenciación de la médula ósea en DC es complicado y costoso. Dos. Aislamiento de médula ósea y preparación de células BM Sacrificamos ratones y fijamos en mesa de operaciones de ratón.

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

Inmunología e infección Número 185 Célula dendrítica médula ósea DC BMDC factor estimulante de colonias de granulocitos-macrófagos interleucina-4