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Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Article DOI: 10.3791/2036-v • 08:56 min • August 1st, 2010
August 1st, 2010 •
1, 1
1Cardiovascular Research Institute, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco

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Directed differentiation of hESCs into specific cells has generated much interest in regenerative medicine. We provide a concise, step-by-step protocol for determining the in vivo fate of selected hESCs that provides a valuable tool for characterizing tissue-specific reagents for cell-based therapy.

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Fate Mapping Human Embryonic Stem Cells Teratoma Formation Self-renewal Differentiation Regenerative Medicine Directed Differentiation In Vivo Fate HESCs Phaseolus Vulgaris Lectin Cell Pellet Kidney Capsule Immunodeficient Mouse Teratoma Size Immunohistochemistry Tissue-specific Reagents Cell-based Therapy
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