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Immunology and Infection

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Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

Article DOI: 10.3791/2355-v • 07:25 min • December 26th, 2010
December 26th, 2010 •
1, 1, 1, 2, 1
1Department of Medical Microbiology and Immunology, University of California, Davis, 2Département Génétique et Développement, Institut Cochin, Université Paris Descartes

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Oligonucleotides can be used to site specifically substitute a single nucleotide of transfected target genes in both Anopheles gambiae and Anopheles stephensi cells.

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Transfection Mutagenesis Target Genes Mosquito Cells Locked Nucleic Acid-modified Oligonucleotides Plasmodium Parasites Malaria Genetically Modified Mosquitoes Cell Signaling Pathways Targeted Mutagenesis Gene Targets Homologous Recombination DNA Molecule Stably Transformed Cell Lines Locked Nucleic Acid-modified Oligonucleotides (LNA-ONs) Artificial Single Nucleotide Substitutions
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