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A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels

Article DOI: 10.3791/3850-v • 05:31 min • April 24th, 2012
April 24th, 2012 •
1, 1, 1
1Department of Pharmacology, Physiology & Neuroscience, University of South Carolina, School of Medicine

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A real-time screening procedure for identifying drugs that interact with G protein-gated inward rectifier K+ (GIRK) channels is described. The assay utilizes membrane potential-sensitive fluorescent dyes to measure GIRK channel activity. This technique is adaptable for use on a number of cell lines.

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Fluorescent Screening Assay Modulators GIRK Channels G Protein-gated Inward Rectifier K+ Cellular Mediators Hormones Neurotransmitters Brain Heart Skeletal Muscle Endocrine Tissue Ligands Plasma Membrane-bound Receptors G Protein-coupled Receptors (GPCRs) Stimulation Of G Proteins Gi And Go K+ Movement Out Of The Cell Resting Membrane Potential Neurons Action Potential Formation Excitatory Neurotransmitters Heart Rate Therapeutic Agents Neuropathic Pain Drug Addiction Cardiac Arrhythmias Pharmacology Anti-arrhythmic Agents Antipsychotic Drugs Antidepressants
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