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Imaging Dendritic Spines in Caenorhabditis elegans
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Imaging Dendritic Spines in Caenorhabditis elegans

Article DOI: 10.3791/62676-v • 09:14 min • September 27th, 2021
September 27th, 2021 •
1, 1,2
1Department of Cell and Developmental Biology, Vanderbilt University, 2Program of Neuroscience, Vanderbilt University

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Dendritic spines are important cellular features of the nervous system. Here live imaging methods are described for assessing the structure and function of dendritic spines in C. elegans. These approaches support the development of mutant screens for genes that define dendritic spine shape or function.

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Dendritic Spines Caenorhabditis Elegans Visualizing Calcium Transients Neurons Genetic Approaches Spine Morphogenesis GABAergic Neurons Immobilizing Image Acquisition Laser Configurations Anesthetic Solution Agarose Pads Coverslip Adhesive Sealant Mixture Super-resolution Acquisition Laser Scanning Confocal Microscope Z-stacks Image Processing Nyquist Acquisition Pixel Size
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