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Pyrosequencing
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Pyrosequencing: A Simple Method for Accurate Genotyping

Article DOI: 10.3791/630-v • 13:07 min • January 8th, 2008
January 8th, 2008 •
1, 1
1Department of Internal Medicine, Washington University in St. Louis

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Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained.

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Pyrosequencing Genotyping Pharmacogenetic Research Human Genome Project Data Genotyping Methods Polymorphisms Tri-allelic Indels Short-repeat Polymorphisms Allele Percentages Methylation Pooled Sample Assessment Internal Quality Control Single-nucleotide Polymorphism Evaluation Clinically Relevant Polymorphisms Technique Methodology
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